Capto Q ImpRes resin couples a quarternary ammonium (Q) strong anion exchanger to a chemically modified, high-flow agarose matrix. Scaling up allows for high resolution combined with quick flow rates. Designed to offer quality and flexibility in process-scale applications.
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InertSustainSwift C18 Capillary Columns, Particle Size 3µm, I.D. Capto adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules.Inertsil ODS-4 Capillary Columns, Particle Size 5µm, I.D.High-throughput medium for intermediate and polishing protein purification, excellent choice for process-scale purification. InertSustainSwift C18 Preparative Columns, Particle Size 5µm, I.D. HiTrap Capto Q ImpRes is a strong anion exchanger prepacked with BioProcess Capto ImpRes media for screening and small-scale protein purifications using ion exchange chromatography (IEX).
![capto impres capto impres](https://assets.fishersci.com/TFS-Assets/CCG/product-images/F186532~p.eps-650.jpg)
Because of poor resolution between the monomer and aggregates, MAb3 could not be purified in flow-through mode on Capto adhere ImpRes, and hence, bind-and-elute mode was evaluated. Capto adhere ImpRes in bind-and-elute mode.
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In this work, with two concrete cases (WuXiBodies with asymmetric and symmetric designs, respectively), we showed that Capto MMC ImpRes mixed-mode chromatography can effectively remove LC-missing byproducts as well as aggregates, demonstrating that this resin is a powerful tool for WuXiBody purification.Īntibody aggregates Bispecific antibody (bsAb) Capto MMC ImpRes Light chain (LC)-missing byproduct Mixed-mode chromatography WuXiBody.Ĭopyright © 2020 Elsevier Inc. The results from Capto SP ImpRes and Capto Q polishing steps are summarized in Table 3. Nevertheless, WuXiBody's unique design greatly facilitates removal of this type of impurity, which can otherwise be difficult to clear. LC-missing species, a byproduct frequently associated with bsAb containing sequence engineered Fab arm, is also identified during WuXiBody production. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. Its key feature is the replacement of one parental antibody's CH1/CL constant region with the T cell receptor (TCR) constant domain, a design aimed at promoting cognate heavy chain (HC)-light chain (LC) pairing. Capto Butyl combines high capacity, high flow rate and low backpressure reduces process cycle times and increases productivity. HiTrap Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto MMC ImpRes weak cation exchange multimodal medium for screening and small-scale protein purifications using ion exchange chromatography (IEX).
![capto impres capto impres](https://us.vwr.com/stibo/bigweb/std.lang.all/17/87/33231787.jpg)
WuXiBody is a novel bispecific antibody (bsAb) platform developed by WuXi Biologics. Capto Butyl is a hydrophobic interaction chromatography (HIC) medium used in the capture and intermediate stages of protein purification. Capto MMC ImpRes is a weak cation exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules.